Up to six players can play, using any controller. The object of the game, as in the board game, is to find out who killed Mr. Boddy where and with what.
Each player is dealt a number of cards. All the cards held eliminate the suspect, weapon, or room on the card. Each player can view their own cards during their turn. A die is rolled, and the six players (represented by the six suspects Miss Scarlet, Col. Mustard, Mrs. White, Mr. Green, Mrs. Peacock, and Prof. Plum) move around the board. Upon entering one of the nine rooms on the board, the player must make a suggestion, choosing a suspect and a weapon (the room the player is in is automatically chosen.) A vignette is shown, showing who or what was in that room, or who held the weapon. This would result in a clue given, such as "Miss Scarlet was in the Lounge" or "No one had the wrench." On the highest difficulty, the clues given are less broad, like in the board game, with clues only given that the chosen suspect or weapon was or wasn't in the room. The suspect chosen in the suggestion is moved to that room.
A player can, on their turn, make an interrogation, wherein they can choose a suspect, a weapon, and a room. A longer vignette is shown, playing out the scenario as the player chose. Example: "The lounge was warm. It was cozy enough for a nap... A long nap. Miss Scarlet laughed as she fixed her hair. She picked up the pipe." At this point, one of the other players, if they are in possession of a card that eliminates any of those factors, shows on screen that they have proof that the scenario could not have happened. If none of the other players are in possession of such a card, the interrogating player is not proved wrong. Players may make two interrogations per game.
A player can, on their turn, make an accusation, which plays out similar to an interrogation, unless if the player is incorrect, they are eliminated from the game. If they are correct, the actual suspect is seen killing Mr. Boddy in an addition to the vignette and is then seen being arrested. The accusing player is the winner.