Spartan: Total Warrior units
Spartans
- Spartan Soldier: Armed with a sword and shield, these warriors make up the backbone of the spartan army.
- Spartan Hoplite: Armed with a spear that gives them a long attack reach, these elite soldiers are the best the spartan army has to offer, they can be easily identified by their crested helmets.
- Spartan Archer: Armed with bows, spartan archers are lethal at range but vulnerable in close combat, they boast superior accuracy to their roman counterparts.
- Sapper: Spartan sappers are engineers who carry explosives to wherever they are needed on the battlefield, once they have deployed and primed their explosives the will draw their sword and help their fellow soldiers in battle.
- Allied Gigantes: Appears only as an Arena Challenge extra.
Romans
- Roman Recruit: Fresh recruits to the Roman army, Roman recruits are only lightly armoured and carry a light shield that can easily be broken.
- Roman Soldier: Roman soldiers make up the backbone of the roman army, more heavily armoured than the recruits they can block attacks much more effectively and are most dangerous in large formations.
- Roman Archer: Armed with a bows, roman archers support the legions from afar.
- Heavy legionary: Elite soldiers of the Roman army. Equivalent to Spartan Swordsmen in terms of skill and durability, they are much more heavily armed than their normal counterparts.
- Centurion: Commanders of the Roman legions, these men lead their cohorts into battle and are dangerous opponents . All of them resemble Marcus the Centurion, the Roman military adviser from Rome: Total War.
Barbarians
- Barbarian Warrior: Armed with a large club. Equivalent to the Roman Recruit.
- Barbarian Raider: Wielding swords. they are equivalent to the Roman Soldier.
- Barbarian Hunter: Armed with throwing axes.
- Berserker: Armed with sharp knives on their hands, they are equivalent to the Centurion. They act as bodyguards to Beowulf.
- Gigantes: Giant creatures that are a threat to any soldier even the spartan.
- Strong Gigantes: A special type of Gigante that, while it has the same strength as a normal one, it is harder to kill because it has more health. The only one of these in the game is one of the 2 Gigantes in "The Wild Bunch" chapter.
Praetorians
- Praetorian Guard: The basic Praetorian soldier.
- Praetorian Archer: Armed with a bow and arrows.
- Praetorian Legionary: A tougher variety of Praetorian Guard.
- Praetorian Carnifex: Strong soldiers armed with long swords which they use as shields.
- Infernus: Armed with a flame thrower.
- Assassin: Fast soldiers that are a challenge to even the Spartan. They can use their twin swords as shields and can evade even direct rage attacks. Somewhat resembles the Arcani unit from Rome: Total War.
Undead
- Undead Warrior: Zombified remains of a Roman Soldier. Will continually return to life unless stomped on or stabbed while on the ground.
- Skeleton Warrior: Skeletal remains of Greek and Trojan warriors who died during the Trojan War, reanimated through sorcery. Impervious to arrow attacks.
- Skeleton Archer: Identical to the Skeleton Warrior, but armed with a bow and arrows.
Gladiators
- Hoplomachus Heavy Axe Gladiator: A large, powerful, heavily armored warrior armed with battle axes.
- Thracian Light Arms Gladiator: Agile, but unarmored, gladiator spearman.
- Retarius Trident Gladiator: Similar to Thracian Light Arms.
Civilians
- Male Villager: Found in the village that is attacked by barbarians. Incapable of combat.
- Female Villager: Found in the village that is attacked by barbarians. Incapable of combat.
- Athenian Male: Resident of Athens. Incapable of combat.
- Athenian Female: Resident of Athens. Incapable of combat.
Heroes and villains
- The Spartan: The player character. On a quest to of revenge against the Romans for attacking Sparta.
- Castor: Armed with a long spear. One of the Spartan's best friends, he accompanies him on his journey to fight the Romans.
- Pollux: Another one of the Spartans friends and Castor's brother. Armed with a sword and shield.
- Electra: Amazon princess rescued by the Spartan and castor from the Roman camp. Aids them in their quest to destroy the Roman Empire.
- King Leonidas: The ruler of Sparta.
- Archimedes: Scientist and leader of the resistance to liberate Athens from Roman rule.
- Talos: Giant mechanical man created by the Roman Empire to destroy Sparta.
- Medusa: Queen of the Gorgons, she has the devastating power to turn men into stone with a single glance. She is captured by the Romans and used in an attempt to destroy Sparta.
- Crassus: Roman general. Armed with Medusa's shield, which is able to turn men to stone.
- Beowulf: Chief of the barbarians. Able to create deadly fire and armed with his warhammer which causes small earthquakes.
- Nemesis: A clone of the Spartan armed with the Spear of Achilles.
- Hydra: A multi-headed serpentine monster which roams the ruins of Troy.
- Sejanus: Praetorian prefect and ruler of Athens following its fall. Able to teleport himself, fire flaming bolts, shield himself from attack, and raise the dead to fight at his command.
- The Minotaur: A large creature with the body of a man and the head of a bull. Captured by the Romans and forced to fight in the coliseum, but he escapes in the sewer.
- Tiberius: Emperor of the Roman Empire.
- Ares': The god of war, he guides the Spartan in his quest.