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The Sega CD version has a password system to continue the game from a set point. The game begins with Doctor Octopus and Electro available in the map. The Lizard and Sandman appear in the map when Dr. Octopus is defeated, and Vulture after defeating Electro. Mysterio appears after Vulture's defeat. After defeating all the six, Hobgoblin appears, followed by Venom and then the Kingpin.
|HALF2LIFE||Dr. Octopus and Electro|
|WALLABY||Electro and Vulture|
|MECHANIC||Dr. Octopus and Sandman|
|TOTALLY2||Dr. Octopus and Lizard|
|GALLON66||Electro, Vulture and Mysterio|
|MONSTER4||Dr. Octopus, Electro and Vulture|
|COWBOY36||Dr. Octopus, Electro and Sandman|
|STABLE8||Dr. Octopus, Electro and Lizard|
|REACTOR5||Dr. Octopus, Sandman and Lizard|
|FALCON499||Dr. Octopus, Electro, Vulture and Mysterio|
|MARBLETOP||Dr. Octopus, Electro, Vulture and Sandman|
|SCHMENGE||Dr. Octopus, Electro, Vulture and Lizard|
|PERMANENT||Dr. Octopus, Electro, Sandman and Lizard|
|HELPINHAND||Dr. Octopus, Electro, Vulture, Mysterio and Sandman|
|SNIGLET7||Dr. Octopus, Electro, Vulture, Mysterio and Lizard|
|NORBLE||Dr. Octopus, Electro, Vulture, Sandman and Lizard|
|PUBLIC45||Dr. Octopus, Electro, Vulture, Mysterio, Sandman and Lizard|